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Image Search Results
Journal: Chemical Science
Article Title: Direct decarboxylation of ten-eleven translocation-produced 5-carboxylcytosine in mammalian genomes forms a new mechanism for active DNA demethylation
doi: 10.1039/d1sc02161c
Figure Lengend Snippet: Decarboxylation of F-5caC is a rapid process and occurs in diverse mammalian cells. (a–c) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of HEK293T cells upon feeding of 300 μM of F-5caC for different times. (d) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of Jurkat-T, MCF-7, and Neuro-2a cells upon feeding of 300 μM of F-5caC for 3 d. (e) Schematic illustration of the active 5mC demethylation through two pathways: the direct decarboxylation of TET-produced 5caC to cytosine in mammalian genomes established in the current study and the previous TET-TDG-BER pathway.
Article Snippet: The human embryonic kidney epithelial cell line (HEK293T),
Techniques: Produced